Method for expression of proteins in bacterial host cells

An aminopeptidase inhibitor is used when expressing heterologous protein in a bacterial host, such as Streptomyces. Use of such an inhibitor inhibits degradation of the heterologous protein by aminopeptidases. Inhibitors are designed based upon the mechanism and substrate specificity of the target p...

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Main Authors HADARY; DANY, WALCZYK; EVA, JENISH; DAVID L, GARVEN; SHEILA, BUTLER; MICHAEL J, KRIEGER; TIMOTHY J, BARTFELD; DANIEL, SOOSTMEYER; GISELA, KRYGSMAN; PHYLLIS, MALEK; LAWRENCE T
Format Patent
LanguageEnglish
Published 03.10.2000
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:An aminopeptidase inhibitor is used when expressing heterologous protein in a bacterial host, such as Streptomyces. Use of such an inhibitor inhibits degradation of the heterologous protein by aminopeptidases. Inhibitors are designed based upon the mechanism and substrate specificity of the target protease and expressed protein.
Bibliography:Application Number: US19970951742