Acidic phospholipase, production and methods using thereof

An acidic phospholipase is obtained from a strain of the genus Hyphozyma. It is able to hydrolyze both fatty acyl groups in intact phospholipid. Advantageously, it has no lipase activity and is active at very low pH; these properties make it very suitable for use in oil degumming, as enzymatic and a...

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Bibliographic Details
Main Authors HALKIER; TORBEN, STRINGER; MARY ANN, HASIDA; MIYOKO, TSUTSUMI; NORIKO
Format Patent
LanguageEnglish
Published 03.10.2000
Edition7
Subjects
ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES
BAKERY PRODUCTS
BAKING
BEER
BIOCHEMISTRY
CANDLES
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DETERGENTS
EDIBLE DOUGHS
ENZYMOLOGY
ESSENTIAL OILS
FATTY ACIDS THEREFROM
FOODS OR FOODSTUFFS
HUMAN NECESSITIES
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PERFUMES
PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
PRESERVATION THEREOF
PRODUCING (PRESSING, EXTRACTION), REFINING AND PRESERVINGFATS, FATTY SUBSTANCES (e.g. LANOLIN), FATTY OILS AND WAXES,INCLUDING EXTRACTION FROM WASTE MATERIALS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
PROTEIN COMPOSITIONS FOR FOODSTUFFS
SPIRITS
THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BYADDITION OF MATERIALS
VINEGAR
WINE
WORKING-UP PROTEINS FOR FOODSTUFFS
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Summary:An acidic phospholipase is obtained from a strain of the genus Hyphozyma. It is able to hydrolyze both fatty acyl groups in intact phospholipid. Advantageously, it has no lipase activity and is active at very low pH; these properties make it very suitable for use in oil degumming, as enzymatic and alkaline hydrolysis (saponification) of the oil can both be suppressed. The phospholipase is not membrane bound, making it suitable for commercial production and purification.
Bibliography:Application Number: US19990295186