Kit for conducting an assay to detect a substance using enzymatically-induced decomposition of dioxetanes
A kit for detecting a first substance in a sample comprising a dioxetane having the formula: wherein T is a cycloalkyl or polycycloalkyl group bonded to the 4-membered ring portion of the dioxetane by a spiro linkage; Y is a fluorescent chromophore; X is H, alkyl, aryl, aralkyl, alkaryl, heteroalkyl...
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Main Author | |
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Format | Patent |
Language | English |
Published |
05.09.2000
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Edition | 7 |
Subjects | |
Online Access | Get full text |
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Summary: | A kit for detecting a first substance in a sample comprising a dioxetane having the formula: wherein T is a cycloalkyl or polycycloalkyl group bonded to the 4-membered ring portion of the dioxetane by a spiro linkage; Y is a fluorescent chromophore; X is H, alkyl, aryl, aralkyl, alkaryl, heteroalkyl, heteroaryl, cycloalkyl, cycloheteroalkyl, or an enzyme-cleavable group; and Z is H or an enzyme-cleavable group, provided that at least one of X or Z must be an enzyme-cleavable group; and an enzyme which cleaves the enzyme-cleavable group of the dioxetane creating an electron-rich moiety which destabilizes the dioxetane, causing it to decompose into two ketones, one ketone comprising the moiety T, and the other ketone comprising moieties X, Y and a portion of Z. The energy released by decomposition causes the moiety Y to luminesce. |
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Bibliography: | Application Number: US19980182416 |