Glucose-6-phosphate dehydrogenase deficient mice and methods of using same

• Main Authors: LONGO; LETIZIA, LUZZATTO; LUCIO
• Format: Patent
• Language: English
• Published: 30.05.2000
• Edition: 7
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

Mice which produce measurable levels of glucose-6-phosphate dehydrogenase (G6PD) deficient red blood cells can be used as animal models to evaluate new drugs for risk of inducing hemolytic anemia in G6PD-deficient individuals, and for pre-clinical evaluation of gene therapy protocols to correct G6PD deficiency. Deficient and wild-type cells can be distinguished using a tetrazolium dye, and the numbers of each type of cell counted before and after exposure of the cells to the drug or therapy.