Target nucleic acid sequence amplification

A method is provided for the rapid, substantially isostatic, segregation and amplification of the sequence information of a target nucleic acid sequence positioned within a single- or double-stranded polynucleotide. The method is based on the serial generation of double-stranded DNA engineered to co...

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Bibliographic Details
Main Authors BLASSAK; MICHELE L, MUELLER; REINHOLD, WILKOSZ; RICHARD K, WICK; JAMES F
Format Patent
LanguageEnglish
Published 16.05.2000
Edition7
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A method is provided for the rapid, substantially isostatic, segregation and amplification of the sequence information of a target nucleic acid sequence positioned within a single- or double-stranded polynucleotide. The method is based on the serial generation of double-stranded DNA engineered to contain terminal nicking sites, nicking of those sites, and extensions from those nicks, thereby displacing any existing polynucleotides. Further provided is a method for detecting polynucleotides using the method of the invention. A kit combining the components commonly used in practicing the method of the invention is also provided.
Bibliography:Application Number: US19970949770