Crystallized formulation for staining DNA, its preparation and kit

• Main Author: BARBESTI; SILVIA
• Format: Patent
• Language: English
• Published: 19.05.1998
• Edition: 6
• Subjects: ADHESIVES; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; DYES; ENZYMOLOGY; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; LAKES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MISCELLANEOUS APPLICATIONS OF MATERIALS; MISCELLANEOUS COMPOSITIONS; MORDANTS; MUTATION OR GENETIC ENGINEERING; NATURAL RESINS; ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES; PAINTS; PHYSICS; POLISHES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION; TECHNICAL SUBJECTS COVERED BY FORMER USPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TESTING; VINEGAR; WINE

PCT No. PCT/EP94/03217 Sec. 371 Date May 10, 1996 Sec. 102(e) Date May 10, 1996 PCT Filed Sep. 24, 1994 PCT Pub. No. WO96/09529 PCT Pub. Date Mar. 28, 1996A process is disclosed for making a crystallized formulation for staining nuclear DNA to be used in flow cytometery. The crystallized DNA staining formulation and a kit containing the crystallized DNA staining formulation are provided. The staining formulation contains a mixture of a dye, a ribonuclease and a non-ionic hydrophilic surfactant. The process for preparing the formulation comprises mixing the dye, the enzyme and the surfactant and evaporating the mixture under vacuum to form a crystallized residue. The kit comprises the crystallized DNA staining formulation packed in single dose vials, each vial being sufficient for one analytic determination. Optionally, the kit may contain additional vials comprising a diluent used to reconstitute the crystallized residue at the time of use.\!