Materials and methods for biosynthesis of serine and serine-related products

PCT No. PCT/EP92/01873 Sec. 371 Date May 24, 1994 Sec. 102(e) Date May 24, 1994 PCT Filed Aug. 17, 1992 PCT Pub. No. WO93/12235 PCT Pub. Date Jun. 24, 1993Engineered DNA encoding 3-phosphoglycerate dehydrogenate (PGD) has reduced sensitivity to inhibition by serine in comparison to wild-type PGD. Th...

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Bibliographic Details
Main Author BURLINGAME; RICHARD P
Format Patent
LanguageEnglish
Published 08.04.1997
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:PCT No. PCT/EP92/01873 Sec. 371 Date May 24, 1994 Sec. 102(e) Date May 24, 1994 PCT Filed Aug. 17, 1992 PCT Pub. No. WO93/12235 PCT Pub. Date Jun. 24, 1993Engineered DNA encoding 3-phosphoglycerate dehydrogenate (PGD) has reduced sensitivity to inhibition by serine in comparison to wild-type PGD. The DNA encodes PGD which has at least some level of enzymatic activity useful for biosynthesis, and which retains that activity at higher serine levels than does the (unmodified) wild-type PGD. The PGD has the amino acid sequence of this above-described engineered DNA. Expression vectors contain this engineered DNA and regulatory DNA positioned and oriented for expression of the engineered DNA in a host expression system. Cells contain these expression vectors, and a method for producing serine or a serine-derived product includes culturing such cells.
Bibliography:Application Number: US19940244491