Release and isolation of N-glycans and O-glycans

N- and O-glycans are released from a glycoconjugate with a substantially anhydrous hydrazine reagent, the glycoconjugate being substantially salt-free and substantially anhydrous. Released N- and O-glycans are recovered in substantially unreduced and intact form using a chromatographic material.

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Bibliographic Details
Main Authors BRUCE; JAMES, PAREKH; RAJESH B, MERRY; ANTHONY H
Format Patent
LanguageEnglish
Published 23.07.1996
Edition6
Subjects
APPARATUS THEREFOR
CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
CHEMISTRY
COMPOSITIONS BASED THEREON
DERIVATIVES THEREOF
GENERAL METHODS OF ORGANIC CHEMISTRY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
ORGANIC MACROMOLECULAR COMPOUNDS
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
PHYSICS
POLYSACCHARIDES
SUGARS
TESTING
THEIR PREPARATION OR CHEMICAL WORKING-UP
TRANSPORTING
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Summary:N- and O-glycans are released from a glycoconjugate with a substantially anhydrous hydrazine reagent, the glycoconjugate being substantially salt-free and substantially anhydrous. Released N- and O-glycans are recovered in substantially unreduced and intact form using a chromatographic material.
Bibliography:Application Number: US19930195761