Enzymatic method for synthesis of cellulose 1

The present invention discloses a method of synthesizing a novel form of cellulose I as well as methods of synthesizing a novel form of cellulose I in vitro. One method comprises contacting an activated saccharide substrate with an endoglucanase in an appropriate organic solvent/buffer ratio. The in...

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Bibliographic Details
Main Authors LI; LIKUN, OKUDA; KAZUO, SHODA; SHIN-ICHIRO, LEE; JONG, KUDLICKA; KRYSTYNA, BROWN, JR.; R. MALCOLM, KUGA; SHIGENORI, KOBAYASHI; SHIRO
Format Patent
LanguageEnglish
Published 05.12.1995
Edition6
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMES
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The present invention discloses a method of synthesizing a novel form of cellulose I as well as methods of synthesizing a novel form of cellulose I in vitro. One method comprises contacting an activated saccharide substrate with an endoglucanase in an appropriate organic solvent/buffer ratio. The invention also encompasses a partially purified endoglucanase and a method of synthesizing cellooligosaccharides. A second method comprises contacting a nucleotide sugar with a purified glycosyl transferase in an appropriate buffer medium to insure polymerization and crystallization of parallel glucan chains from the enzyme/micelle complex to form cellulose I.
Bibliography:Application Number: US19930100868