Alkaline protease Bacillus sp. JP 395, method of making and detergent compositions

PCT No. PCT/DK92/00105 Sec. 371 Date Sep. 23, 1993 Sec. 102(e) Date Sep. 23, 1993 PCT Filed Apr. 3, 1992 PCT Pub. No. WO92/17578 PCT Pub. Date Oct. 15, 1992.An alkaline protease from a strain of Bacillus sp. JP 395 has been isolated. The protease has a mass of about 30 kDa as determined by SDS-PAGE,...

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Bibliographic Details
Main Authors OUTTRUP; HELLE, AASLYNG; DORRIT A, DAMBMANN; CLAUS, CHRISTIANSEN; MARGRETHE
Format Patent
LanguageEnglish
Published 14.11.1995
Edition6
Subjects
ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES
BEER
BIOCHEMISTRY
CANDLES
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DETERGENT COMPOSITIONS
DETERGENTS
ENZYMOLOGY
FATTY ACIDS THEREFROM
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
RECOVERY OF GLYCEROL
RESIN SOAPS
SOAP OR SOAP-MAKING
SPIRITS
USE OF SINGLE SUBSTANCES AS DETERGENTS
VINEGAR
WINE
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Summary:PCT No. PCT/DK92/00105 Sec. 371 Date Sep. 23, 1993 Sec. 102(e) Date Sep. 23, 1993 PCT Filed Apr. 3, 1992 PCT Pub. No. WO92/17578 PCT Pub. Date Oct. 15, 1992.An alkaline protease from a strain of Bacillus sp. JP 395 has been isolated. The protease has a mass of about 30 kDa as determined by SDS-PAGE, a pH optimum of about 9-11, a temperature optimum of about 50 DEG -65 DEG C., and an isoelectric point above 9.5. The protease is inhibited by PMSF (phenylmethylsulfonylfluoride), but not by EDTA or soybean protein inhibitor. The protease is prepared by growing this strain of bacillus in a suitable media and recovering the protease. The protease has been formulated in detergents and is stable at 40 DEG C. for 60 minutes in these detergents under wash conditions in the presence or absence of bleaches such as perborate.
Bibliography:Application Number: US19930119193