Method for determining analyte concentration by cross-reactivity profiling

• Main Authors: AMBLER; STUART M, KAUVAR; LAWRENCE M
• Format: Patent
• Language: English
• Published: 16.08.1994
• Edition: 5
• Subjects: CALCULATING; COMPUTING; COUNTING; ELECTRIC DIGITAL DATA PROCESSING; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The precision of identification of analyte composition in a sample, where the possible analytes cross-react with specific binding reagents, is enhanced by applying pattern recognition techniques. Samples to be tested are reacted with a panel of specific binding reagents reactive with the set of analytes to be tested to obtain a pattern of reactivity with respect to each analyte at a given concentration. This results in a databank of "CRIM profiles" for known concentrations of each analyte. This databank is stored in a computationally accessible form, which then can be matched against CRIM patterns obtained by testing unknown samples. In one embodiment, each CRIM pattern obtained is plotted as a single point in n-dimensional space, wherein n represents the number of specific binding reagents in the panel. Because a different CRIM profile as a function of concentration is obtained with each analyte, a pattern of points in n-dimensional space can be constructed for various concentrations of each individual member. Mathematical techniques can be used to project the pattern from n-dimensional space to reduce dimensionality. The location of a point yielded by a sample of unknown concentration can then be matched against those for predetermined known compositions to determine best estimate.