Detergent comprising isolated cellulase from bacillus ferm bp-3431 or a mutant strain thereof, surfactant and builder

• Main Authors: SAITO; KIYOSHI, MASATSUJI; EIKO, SEKO; MASAHIKO
• Format: Patent
• Language: English
• Published: 24.05.1994
• Edition: 5
• Subjects: ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES; APPARATUS THEREFOR; BEER; BIOCHEMISTRY; CANDLES; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DETERGENT COMPOSITIONS; DETERGENTS; ENZYMOLOGY; FATTY ACIDS THEREFROM; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PAPER-MAKING; PROCESSES USING MICROORGANISMS; PRODUCTION OF CELLULOSE; PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCESFROM CELLULOSE-CONTAINING MATERIALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; RECOVERY OF GLYCEROL; REGENERATION OF PULPING LIQUORS; RESIN SOAPS; SOAP OR SOAP-MAKING; SPIRITS; TEXTILES; USE OF SINGLE SUBSTANCES AS DETERGENTS; VINEGAR; WINE

A cellulase having the following properties: (1) an optimum pH of from 9.5 to 10.5 as measured using carboxymethylcellulose as a substrate; (2) a stable pH of from 6 to 11 as measured using carboxymethylcellulose as a substrate after treating at 30 DEG C. and for 30 minutes; (3) an optimum temperature of about 55 DEG C. as measured using carboxymethylcellulose as a substrate; (4) influence of a surfactant being such that residual activity is 90% or more after treatment at 30 DEG C. and pH of 9.0 for 2 hours in the presence of a sodium n-alkylbenzenesulfonate; (5) a molecular weight of 52,000+/-2,000 as measured by electrophoresis on SDS-polyacrylamide gel; and (6) an isoelectric point of 4.2+/-0.2 as measured by isoelectric electrophoresis. The cellulase is produced by a Bacillus strain FERM BP-3431 or a mutant strain thereof. A detergent comprising the cellulase, a builder and a surfactant is also disclosed.