Octanucleotide restriction endonuclease, Srf I, and method for producing the same

This invention provides restriction endonuclease, Srf I, capable of recognizing the eight nucleotide base palindromic sequence shown below on a double-stranded DNA molecule and cleaving the DNA chain at the asterisk-marked position resulting in blunt ends 5' GCCC*GGGC 3' 3' CGGG*CCCG...

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Bibliographic Details
Main Authors SIMCOX; TIMOTHY G, SIMCOX; MARY E
Format Patent
LanguageEnglish
Published 05.04.1994
Edition5
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC
GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TECHNICAL SUBJECTS COVERED BY FORMER USPC
TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ARTCOLLECTIONS [XRACs] AND DIGESTS
VINEGAR
WINE
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Summary:This invention provides restriction endonuclease, Srf I, capable of recognizing the eight nucleotide base palindromic sequence shown below on a double-stranded DNA molecule and cleaving the DNA chain at the asterisk-marked position resulting in blunt ends 5' GCCC*GGGC 3' 3' CGGG*CCCG 5' (wherein C and G respectively represent cytosine and guanine). The restriction endonuclease is produced by culturing Streptomyces rf in a culture medium and recovering it from the culture.
Bibliography:Application Number: US19930065078