Microbiological process for preparing 9 alpha -hydroxy-4-androstene-3,17-dione

The invention relates to a process for the production of 9 alpha -hydroxy-4-androstene-3,17-dione from natural sterols of plant or animal origin or from mixtures thereof by the submerged aerobic fermentation of an enzyme-deficient, sterol-de-grading microorganism in a nutrient medium containing util...

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Bibliographic Details
Main Authors HANTOS; GABOR, ALBRECHT; KAROLY, MORAVCSIK; IMRE, ILKOEY; EVA, KOENCZOEL; KALMAN, JEKKEL NEE BOKANY; ANTONIA, VIDA; ZSUZESANNA, SZABO; ISTVAN M, LANG; TIBOR, CSAJAGI; EVA, AMBRUS; GABOR, LENGYEL NEE SZEMENYEI; ZSUZSANNA, SIMONOVITS; EMILIA
Format Patent
LanguageEnglish
Published 02.04.1991
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to a process for the production of 9 alpha -hydroxy-4-androstene-3,17-dione from natural sterols of plant or animal origin or from mixtures thereof by the submerged aerobic fermentation of an enzyme-deficient, sterol-de-grading microorganism in a nutrient medium containing utilizable carbon and nitrogen sources as well as mineral salts, and by isolating the product formed, which comprises transforming the sterol or sterol mixture with the culture of a 1,2-steroid-dehydrogenase deficient strain of new Mycobacterium roseum species which is able to degrade the side-chain of natural sterols, preferably with Mycobacterium roseum sp. nov. 1108/1 deposited at the National Collection of Agricultural and Industrial Microorganisms, Budapest, Hungary under the number NCAIM B (P) 000339, and by isolating and purifying the 9 alpha -hydroxy-4-androstene-3,17-dione obtained.
Bibliography:Application Number: US19870116872