Method for derivitization of polynucleotides

A method for the preparation of a 3' end functionalized polynucleotide is disclosed. An amine-functionalized solid phase support is treated sequentially with an anhydride, then with an omega -hydroxylamine. A polynucleotide is chemically synthesized on the treated support and is subsequently cl...

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Bibliographic Details
Main Author STABINSKY; YITZHAK
Format Patent
LanguageEnglish
Published 19.04.1988
Edition4
Subjects
CHEMISTRY
DERIVATIVES THEREOF
METALLURGY
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
SUGARS
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Summary:A method for the preparation of a 3' end functionalized polynucleotide is disclosed. An amine-functionalized solid phase support is treated sequentially with an anhydride, then with an omega -hydroxylamine. A polynucleotide is chemically synthesized on the treated support and is subsequently cleaved therefrom by hydrolysis of the amide bonds. A polynucleotide having a 3' free primary amine is recovered for use in hybridization assays and other uses.
Bibliography:Application Number: US19850744798