Assaying peptidase enzyme activity

A method for assay of enzyme activity which comprises treating an amide compound of the formula with peptidase; treating thus-liberated amine of the formula with an oxidase which consume oxygen and forms pigment by oxidative condensation of said amine with a coupler of the formula and quantitatively...

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Bibliographic Details
Main Authors WATANABE; SUSUMU, HIRATA; TSUTOMU, TAKAHASHI; KIYOHARU, KAGIMOTO; YOSHITAKA, OHTSUKA; AKIRA, MATSUMOTO; KUNIO
Format Patent
LanguageEnglish
Published 23.06.1987
Edition4
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:A method for assay of enzyme activity which comprises treating an amide compound of the formula with peptidase; treating thus-liberated amine of the formula with an oxidase which consume oxygen and forms pigment by oxidative condensation of said amine with a coupler of the formula and quantitatively measuring the detectable changes. Also disclosed is an assay method which comprises treating an amide compound of the formula or a salt thereof, with peptidase to liberate an aniline derivative of the formula oxidizing said aniline derivative; and quantitatively measuring detectable change.
Bibliography:Application Number: US19850713242