METHOD FOR EXTRACTING EXOSOME

The present application relates to the technical field of biomembranes and, in particular, to a method for extracting an exosome. The method for extracting an exosome includes the following steps: step 1. adding a whole blood sample onto a microfluidic centrifuge disk; step 2. separating the whole b...

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Bibliographic Details
Main Authors XU, Zhenyu, Lin, Xiaodong, Chen, Yingna, Chen, Dishen
Format Patent
LanguageEnglish
Published 10.10.2024
Subjects
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
SEPARATION
TRANSPORTING
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Summary:The present application relates to the technical field of biomembranes and, in particular, to a method for extracting an exosome. The method for extracting an exosome includes the following steps: step 1. adding a whole blood sample onto a microfluidic centrifuge disk; step 2. separating the whole blood to obtain plasma; step 3. filtering the plasma with a microfiltration membrane; step 4. mixing the filtered plasma with a loading solution to obtain a mixed solution; step 5. filtering the mixed solution with a surface-modified membrane to trap the exosome on the surface-modified membrane; and step 6. eluting the exosome from the surface-modified membrane to obtain a target exosome.
Bibliography:Application Number: US202418749708