METHODS AND COMPOSITIONS OF MATTER FOR INERT BIOENGINEERING OF A BIOLOGICAL ENTITY

A bioengineering method which comprises introducing an inert nucleic acid cassette into a biological entity without introducing or modifying characteristics or traits in the biological entity. The method comprises receiving or providing a sample comprising the biological entity having a nucleic acid...

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Bibliographic Details
Main Authors BIZ, Alessandra, BORG, Michael, FRIEDBERG, Jeremy N, SINGER, David
Format Patent
LanguageEnglish
Published 15.08.2024
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Summary:A bioengineering method which comprises introducing an inert nucleic acid cassette into a biological entity without introducing or modifying characteristics or traits in the biological entity. The method comprises receiving or providing a sample comprising the biological entity having a nucleic acid sequence; selecting an integration site in the nucleic acid sequence for inserting the inert nucleic acid cassette; designing the inert cassette with optimized primer sequences, optimized probe sequences, optimized stop codons and disrupted start codons, and inserting the inert nucleic acid cassette into the biological entity at the integration site; and validating that no characteristics have been added or modified in the biological entity.
Bibliography:Application Number: US202218564779