CONCENTRATION AND DIAFILTRATION OF OLIGONUCLEOTIDES

A method for concentration of oligonucleotides from a solution comprising negatively charged oligonucleotides is provided. The method includes the steps of: circulating the solution through an ultrafiltration or nanofiltration unit having a membrane, filtering the solution through the membrane to re...

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Bibliographic Details
Main Authors KRISHNAMURTHY, Anand Kumar, BARTELS, Craig R, KELLER, Karsten, YAMAGUCHI, Taisuke
Format Patent
LanguageEnglish
Published 04.07.2024
Subjects
CHEMISTRY
DERIVATIVES THEREOF
METALLURGY
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
SEPARATION
SUGARS
TRANSPORTING
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Summary:A method for concentration of oligonucleotides from a solution comprising negatively charged oligonucleotides is provided. The method includes the steps of: circulating the solution through an ultrafiltration or nanofiltration unit having a membrane, filtering the solution through the membrane to remove salts from the solution and obtain a retentate solution including the oligonucleotides and a permeate solution including the removed salts, diafiltering the retentate solution with a diafiltration buffer to produce a concentrated oligonucleotide solution, and collecting the concentrated oligonucleotide solution. The membrane has a nominal molecular weight cutoff in the range of from about 700 to about 5000 daltons, a negatively charged surface with a zeta potential of −20 mV or lower, and a water flux in the range of 800 to 1500 ml/min/m2.
Bibliography:Application Number: US202118288754