REAL-TIME ADAPTIVE METHODS FOR SPECTRALLY RESOLVING FLUOROPHORES OF A SAMPLE AND SYSTEMS FOR SAME

• Main Authors: Mage, Peter, Bai, Wenyu, Owsley, Keegan
• Format: Patent
• Language: English
• Published: 13.06.2024
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

Aspects of the present disclosure include methods for spectrally resolving light from fluorophores having overlapping fluorescence spectra in a sample using adaptive measurement variance. Methods according to certain embodiments include detecting light with a light detection system from particles of a sample comprising a plurality of fluorophores having overlapping fluorescence spectra, determining measurement variance in the detected light for each particle and spectrally resolving light from each fluorophore in the sample with a weighted least squares algorithm that uses the measurement variance determined for each particle. In some embodiments, the spectral unmixing matrix is calculated by the weighted least squares algorithm using an adaptive measurement variance model. Systems and integrated circuit devices (e.g., a field programmable gate array) for practicing the subject methods are also described. Non-transitory computer readable storage medium are also provided.