METHOD, DEVICE AND COMPUTER PROGRAM FOR DETERMINING A POSITION OF AT LEAST ONE EMITTER IN A SAMPLE

The invention relates to a method for determining a position of at least one emitter in a sample, wherein according to a measuring parameter, the sample is illuminated by activation light, the sample is illuminated by an excitation light intensity distribution comprising a local minimum flanked by m...

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Bibliographic Details
Main Authors SCHMIDT, Roman, DONNERT, Gerald
Format Patent
LanguageEnglish
Published 06.06.2024
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:The invention relates to a method for determining a position of at least one emitter in a sample, wherein according to a measuring parameter, the sample is illuminated by activation light, the sample is illuminated by an excitation light intensity distribution comprising a local minimum flanked by maxima, the excitation light intensity distribution is positioned at a plurality of probing positions determined based on an initial position value, an emission signal from the at least one emitter is detected for each of the probing positions, at least one final position value is determined from the emission signals and the associated probing positions, and a position of the emitter is estimated based on the at least one final position value, wherein the measuring parameter is adjusted based on an adjusted speed parameter. The invention further relates to a device and a computer program for implementing the method.
Bibliography:Application Number: US202218284741