CELL CULTURE METHOD, CELL CULTURE CONTAINER, METHOD FOR PRODUCING CELL CULTURE CONTAINER, AND CELL-CONTAINING STRUCTURE

A cell culture method includes performing adherent culture of cells on a coat layer layered on a surface of a cell culture container by placing a culture medium and the cells in the cell culture container. The coat layer includes a layer (i) that includes a layer (i-1) including gelatin or casein an...

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Main Authors Shionoiri, Momoko, Nakayama, Tomoaki, Sameshima, Tatsuya, Koshizuka, Shinnosuke, Aratani, Tomoyuki, Kaga, Yuki, Ijichi, Rie, Kitazawa, Tomofumi
Format Patent
LanguageEnglish
Published 09.05.2024
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:A cell culture method includes performing adherent culture of cells on a coat layer layered on a surface of a cell culture container by placing a culture medium and the cells in the cell culture container. The coat layer includes a layer (i) that includes a layer (i-1) including gelatin or casein and a layer (i-2) that includes a polycationic material and a layer (ii) that is layered on the layer (i) and includes a cell adhesion factor. The layer (ii) is disposed at a position at which the layer (ii) comes into contact with the cells, or the coat layer includes the layer (i) and the layer (i) is disposed at a position at which the layer (i) comes into contact with the cells, and the culture medium includes the cell adhesion factor.
Bibliography:Application Number: US202418412130