METHOD OF OBTAINING A PHARMACEUTICAL AGENT USED FOR INHIBITING THE PROLIFERATION OF TUMOR CELLS

• Main Authors: LOBANOVA, Galina Leonidovna, EVTINA, Anastasia Alekseevna, YURMAZOVA, Tatiana Alexandrovna, BULDAKOV, Mikhail Alexandrovich, PUSTOVALOV, Alexey Vitalievich, SHIYAN, Lyudmila Nikolaevna, ZOTOV, Fedor, SHVARTSMAN, Dmitry Igorevich
• Format: Patent
• Language: English
• Published: 29.02.2024
• Subjects: CASTING; HUMAN NECESSITIES; HYGIENE; MAKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MEDICAL OR VETERINARY SCIENCE; PERFORMING OPERATIONS; POWDER METALLURGY; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; TRANSPORTING; WORKING METALLIC POWDER

The given invention is related to the field of medicine, namely to experimental research in oncology, and can be used to obtain a pharmaceutical agent for inhibiting the proliferation of HeLa cervical cancer cells. It entails the use of a metal powder obtained in a gaseous medium by means of an electric explosion of a wire made of low-carbon steel carried out with a specific energy of 7-18 kJ/g and a pulse duration of 1.2-2 μs. The explosion is carried out inside a reactor that is pre-evacuated to a residual pressure of 10−2 Pa, and then filled with carbon monoxide to a pressure of 105 Pa at a gas circulation rate flow of 10 m/s. The products of the explosion deposited in a hopper are passivated in air for at least 48 hours. The resulting powder is extracted and mixed with a solution of the RPMI-1640 nutrient medium with L-glutamine, a pH of 7.2, in the proportion of the mass of the powder to the volume of the specified solution from 1:10 to 2.7:10. The solution is then centrifuged until the phases are separated. The liquid phase is decanted and used as a pharmaceutical agent. The purpose of the given invention is to expand the arsenal of tools for inhibiting the proliferation of tumor cells.