FlmG-Dependent Soluble Protein O-Glycosylation Systems In Bacteria

The invention relates to a polynucleotide flmG which encodes Flagellin Modification Protein (FlmG) having a glycosyltransferase activity as well as a recombinant expression vector for bacterial expression comprising the polynucleotide flmG of the invention. Also provided is a prokaryotic protein gly...

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Bibliographic Details
Main Authors KINT, Nicolas, ARDISSONE, Silvia, VIOLLIER, Patrick
Format Patent
LanguageEnglish
Published 01.02.2024
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to a polynucleotide flmG which encodes Flagellin Modification Protein (FlmG) having a glycosyltransferase activity as well as a recombinant expression vector for bacterial expression comprising the polynucleotide flmG of the invention. Also provided is a prokaryotic protein glycosylation kit for soluble O-based glycosylation comprising a bacterial Gram-negative host expressing at least one copy of the recombinant expression vector of the invention and a process for O-glycosylation of soluble proteins of interest.
Bibliography:Application Number: US202118031684