Methods and Systems for Extraction, Processing, and Detection of Nucleic Acids

A removable cartridge to be used in a system for extracting and detecting nucleic acids from heterogeneous samples includes a plurality of reservoirs defining at least a first wash buffer reservoir for holding a first wash buffer and a microfluidic assembly configured to attach to the plurality of r...

Full description

Saved in:
Bibliographic Details
Main Authors LEWIS, Diana J, ROSENBERGER, Erin, URBAN, Joseph Neil, FIERING, Jason, Kim, Ernest, LISSANDRELLO, Charles A, Bell, Caleb R, Chleirigh, Cait Ni, NAIK, Aditi R
Format Patent
LanguageEnglish
Published 04.01.2024
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:A removable cartridge to be used in a system for extracting and detecting nucleic acids from heterogeneous samples includes a plurality of reservoirs defining at least a first wash buffer reservoir for holding a first wash buffer and a microfluidic assembly configured to attach to the plurality of reservoirs. The microfluidic assembly includes at least one sample reservoir and a nucleic acid extraction matrix in fluid communication to an automated sample preparation (ASP) reservoir through a first flow channel defined by the microfluidic assembly. An assay chamber is in fluid communication with a third flow channel and with the waste reservoir through a fourth flow channel such that a labeled nucleic acid-containing sample flows through the assay chamber and then to the waste reservoir, wherein vibration-driven mixing agitates fluids while present in the assay chamber. Finally, a nucleic acid-detecting microarray module is positioned in the assay chamber.
Bibliography:Application Number: US202318141553