Systems and Methods for Digital, Multiplexed, Extracellular Vesicle-Derived Biomarker Diagnostic Lab-on-a-Chip

• Main Authors: Harris, Tyler Lee, Heineck, Daniel Philip, Young, Richard Louis, Murray, Amber Noelle, Saalfeld, Jordan Alexandra, Rice, Bryan Joseph
• Format: Patent
• Language: English
• Published: 28.12.2023
• Subjects: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLIDMATERIALS OR FLUIDS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROMSOLID MATERIALS OR FLUIDS; MEASURING; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; PHYSICS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS; SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATICTABLES OR JIGS; TESTING; TRANSPORTING

In one embodiment, a method for isolating and detection one or more biomarkers of interest on a dielectrophoresis device includes receiving a first biological sample containing one or more biomarkers of interest onto a dielectrophoresis (DEP) electrode array, applying a DEP force through particular electrodes of the DEP electrode array, wherein a strength, direction, and period of time of the DEP force is specific to the one or more biomarkers of interest of the first biological sample, and determining, via a digital sensor, a quantity of the one or more biomarkers of interest of the first biological sample.