METHOD FOR CLASSIFYING WHITE BLOOD CELLS INTO SUBPOPULATIONS

• Main Author: TOYA, Yuji
• Format: Patent
• Language: English
• Published: 26.10.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

Disclosed is a method for classifying white blood cells into subpopulations, comprising: preparing a measurement sample by mixing a specimen containing white blood cells, a hemolysis reagent containing a surfactant, a first fluorescent dye, and a second fluorescent dye; irradiating particles in the measurement sample with light, and detecting optical information including first fluorescence information based on fluorescence from the first fluorescent dye, second fluorescence information based on fluorescence from the second fluorescent dye, and scattered light information; sorting a cell population containing basophils from particles in the measurement sample based on optical information including the first fluorescence information; classifying white blood cells contained in the cell population into subpopulations based on the second fluorescence information and the scattered light information; and counting cells classified as a population of basophils in the subpopulation; wherein the second fluorescent dye is a fluorescent dye having maximum absorption in a wavelength range different from that of the first fluorescent dye.