MICROFLUIDIC CHIP FOR HIGH-THROUGHPUT CELL PAIRING AND FUSION

Microfluidic chips, systems, and methods of making and using thereof are described. The microfluid chip includes a disc-shaped transparent layer containing a plurality of cell traps, pillars, and filers, and a support layer attached thereto. The microfluid chip has at least one inlet port in center...

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Bibliographic Details
Main Authors Aeinehvand, Mohammadmahdi, Martinez-Chapa, Sergio Omar, Voldman, Joel
Format Patent
LanguageEnglish
Published 12.10.2023
Subjects
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
CHEMISTRY
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
SPIRITS
TRANSPORTING
VINEGAR
WINE
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Summary:Microfluidic chips, systems, and methods of making and using thereof are described. The microfluid chip includes a disc-shaped transparent layer containing a plurality of cell traps, pillars, and filers, and a support layer attached thereto. The microfluid chip has at least one inlet port in center of the transparent layer for receiving a sample of liquid and cells, and optionally a plastic cover. The microfluid chip can be designed to be suitable for the forces used for cell pairing/fusion in stationary and spinning format, or suitable for a particular cell fusion method such chemical and electrical methods. The microfluid chip is particularly suited for fusing dendritic cells and tumor cells for immunotherapy, or for generating hybridoma.
Bibliography:Application Number: US202318166767