Methods for Characterization of Viral Genome Using Base Modifications

Methods for assaying a sample of viral particles comprising a single-stranded DNA genome for strand-specificity are disclosed. In embodiments, the methods include synthesis of a synthetic DNA strand complementary to the single-stranded DNA genome by incorporating a modified base to yield a labeled d...

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Bibliographic Details
Main Authors Davis, Steven, Richardson, Jared
Format Patent
LanguageEnglish
Published 21.09.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Methods for assaying a sample of viral particles comprising a single-stranded DNA genome for strand-specificity are disclosed. In embodiments, the methods include synthesis of a synthetic DNA strand complementary to the single-stranded DNA genome by incorporating a modified base to yield a labeled double-stranded DNA product, purification of the labeled double-stranded DNA product, sequencing of the purified double-stranded DNA product on a sequencer, and determining the specificity of the viral single-stranded DNA genome based on the detection of the modified base of the synthetic DNA strand.
Bibliography:Application Number: US202318122246