METHOD AND APPARATUS FOR LOCALIZING OBJECTS BY MEANS OF A LIGHT SHEET

Techniques an apparatuses for localizing objects of a sample that emit detection radiation are disclosed. At least one light sheet is produced in a light sheet plane and a region of the sample to be imaged is illuminated with the light sheet and detection radiation from the sample is spatially resol...

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Bibliographic Details
Main Authors Kalkbrenner, Thomas, Siebenmorgen, Jörg
Format Patent
LanguageEnglish
Published 10.08.2023
Subjects
OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
OPTICS
PHYSICS
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Summary:Techniques an apparatuses for localizing objects of a sample that emit detection radiation are disclosed. At least one light sheet is produced in a light sheet plane and a region of the sample to be imaged is illuminated with the light sheet and detection radiation from the sample is spatially resolved in a detection plane. A light sheet is produced that has, in the light sheet plane, a non-illuminated light sheet section delimited by an illuminated light sheet section or is produced sequentially in the light sheet plane, where a non-illuminated light sheet section remains between the sequentially produced light sheets. An object to be localized is present in the non-illuminated region, and a position of the object is captured if it leaves the non-illuminated region and is excited to emit the detection radiation by one of the light sheets delimiting the non-illuminated region.
Bibliography:Application Number: US202318166773