ANTIBODY MANUFACTURE

Described herein is a method for assessing disulfide bond reduction potential of a protein of interest comprising the following steps:Providing a cell culture fluid sample comprising mammalian cells expressing a protein of interest at a concentration within the range of between 0.2 g/l to 10 g/lFilt...

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Bibliographic Details
Main Authors LIU, Yi, THAI, Vu, TJANDRA, Hendri
Format Patent
LanguageEnglish
Published 13.07.2023
Subjects
CHEMISTRY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PHYSICS
TESTING
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Summary:Described herein is a method for assessing disulfide bond reduction potential of a protein of interest comprising the following steps:Providing a cell culture fluid sample comprising mammalian cells expressing a protein of interest at a concentration within the range of between 0.2 g/l to 10 g/lFiltering said cell culture fluid sample over at least one filterDisplacing O2 in the filtered cell culture fluid sampleCollecting at least one sample of the O2-displaced filtered cell culture fluid sampleSeparating the proteins in said at least one O2-displaced, filtered cell culture fluid sample according to their size under non-denaturing conditionsDetermining the disulfide bond reduction potential of the protein of interest.
Bibliography:Application Number: US202318154386