METHODS AND SYSTEMS FOR PROCESSING POLYNUCLEOTIDES

• Main Authors: Lucero, Michael Ybarra, O'Keeffe, Christopher Joachim, Mikkelsen, Tarjei Sigurd, McDermott, Geoffrey, Taylor, Sarah, Ryvkin, Paul, Wu, Indira, Belgrader, Phillip, Pfeiffer, Katherine, Lenji, Mohammad Rahimi, Boutet, Stephane Claude, Bent, Zachary, Srinivas, Niranjan, Stuelpnagel, John R, Bharadwaj, Rajiv, Gopalan, Vijay Kumar Sreenivasa, Meer, Elliott, Price, Andrew D, Wheeler, Tobias Daniel, Saxonov, Serge, Terry, Jessica Michele, Ziraldo, Solongo Batjargal, Hindson, Christopher, Harada, Josephine
• Format: Patent
• Language: English
• Published: 29.06.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for "tagging" analytes for characterization.