METHOD FOR BREEDING NEW GERMPLASM OF CLUBROOT-RESISTANT ORANGE-HEADING CHINESE CABBAGE

A method for breeding a new germplasm of clubroot-resistant orange-heading Chinese cabbage includes: (1) obtaining F1 by hybridizing orange-heading Chinese cabbage as a female parent and clubroot-resistant Chinese cabbage as a male parent, obtaining F2 by selfing F1 individual plants, planting F2 po...

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Bibliographic Details
Main Authors MENG, Yan, LU, Qianqian, HE, Yuting, ZHANG, Lugang, ZHANG, Ninan, WANG, Yaxiu, ZHANG, Xuefen, LIU, Xiaoyuan
Format Patent
LanguageEnglish
Published 22.06.2023
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
TRAPPING
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Summary:A method for breeding a new germplasm of clubroot-resistant orange-heading Chinese cabbage includes: (1) obtaining F1 by hybridizing orange-heading Chinese cabbage as a female parent and clubroot-resistant Chinese cabbage as a male parent, obtaining F2 by selfing F1 individual plants, planting F2 populations, and extracting DNA from the individual plants of F2; (2) observing horticultural traits of F2, and performing observation and statistics of head color phenotypic traits through cutting head; (3) performing PCR amplification with DNA of F2 using a dominant orange gene marker Br530 and a clubroot-resistant marker SC2930-T/SC2930Q, and identifying a genotype of the individual plants; (4) performing a comprehensive evaluation according to the results of marker detection and the observation of horticultural traits, selecting double-site homozygous clubroot-resistant orange-heading Chinese cabbage plants, and selfing the individual plants for 2 consecutive generations; and (5) obtaining a new germplasm of clubroot-resistant orange-heading Chinese cabbage after selfing for 3 generations.
Bibliography:Application Number: US202218047286