METHOD FOR TREATING HIGHLY ACTIVE NK CELLS

The object is to provide a method for cryopreserving and thawing cells with maintaining high viability and high activity, which is applicable to highly active NK cells. A method for treating cells, which comprises the following steps of: (1) collecting in vitro-activated cells in a medium for cell c...

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Bibliographic Details
Main Authors Harada, Yui, Yonemitsu, Yoshikazu
Format Patent
LanguageEnglish
Published 13.04.2023
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PEST REPELLANTS OR ATTRACTANTS
PLANT GROWTH REGULATORS
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TRAPPING
VINEGAR
WINE
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Summary:The object is to provide a method for cryopreserving and thawing cells with maintaining high viability and high activity, which is applicable to highly active NK cells. A method for treating cells, which comprises the following steps of: (1) collecting in vitro-activated cells in a medium for cell culture; (2) suspending the collected cells in a solution for cryopreservation; and (3) freezing the suspended cells. The step (1) preferably includes a treatment with a medium supplemented with any selected from the group consisting of a bile acid and phenylbutyric acid.
Bibliography:Application Number: US202117908472