METABOLOMICS RELATIVE QUANTITATIVE ANALYSIS METHOD BASED ON UPLC/HMRS

• Main Authors: Gu, Congshun, Dai, Zhenli, Ren, Jianhong, Xie, Guigang, Xu, Ying, Gu, Mengnan
• Format: Patent
• Language: English
• Published: 30.03.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The present invention belongs to the technical field of metabolomics, and relates to a metabolomics relative quantitative analysis method based on UPLC/HRMS. Specifically, the present method mainly comprises the steps of formulating an isotope internal standard mixed solution, a standard curve correction solution, and a metabolomics sample solution; acquiring raw mass spectrum data of the standard curve correction solution and the metabolomics sample solution; transposing and deconvolving the raw data; identifying and selecting the optimal isotope internal standard; fitting a linear equation and calculating the relative quantitative results of metabolites in the metabolomics sample solution; and completing the structural identification of metabolites and differential metabolites in the metabolomics sample solution. The method can meet both qualitative and quantitative requirements using only a high-resolution mass spectrometry platform; the quantitative results are accurate, and the accuracy of the qualitative results are higher, having low costs, simple operation, and wide applicability.