Method and apparatus for detecting a presence of a fluorescence pattern type on an organ section by means of immunofluorescence microscopy

• Main Authors: Hahn, Melanie, Hocke, Jens, Krause, Christopher, Voigt, Joern, Krauth, Jens, Gerlach, Stefan, Marzahl, Christian
• Format: Patent
• Language: English
• Published: 22.12.2022
• Subjects: CALCULATING; COMPUTER SYSTEMS BASED ON SPECIFIC COMPUTATIONAL MODELS; COMPUTING; COUNTING; IMAGE DATA PROCESSING OR GENERATION, IN GENERAL; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

A method for detecting at least one potential presence of at least one fluorescence pattern type on an organ section by means of immunofluorescence microscopy and by means of digital image processing is disclosed. An organ section is provided on a slide and the section is incubated with a liquid patient sample which potentially includes primary antibodies and with secondary antibodies which have been labelled with a fluorescent dye. A fluorescence image of the organ section is acquired. A determination is made, by segmentation of the fluorescence image by means of a first neural network, of a sub-area of the fluorescence image that is relevant to formation of the fluorescence pattern type. A determination is made, on the basis of the fluorescence image by means of a second neural network, of the measure of confidence that indicates an actual presence of the fluorescence pattern type. A determination is made, on the basis of the previously determined sub-area, of validity information that indicates a degree of a validity of the measure of confidence. And the measure of confidence of the actual presence of the fluorescence pattern type and of the validity information is outputted.