Multiphoton Imaging Methods in a Scattering and/or Absorbing Medium, and Articles

• Main Authors: Hansen, James D, Ender, David A, Hughes, Gareth A, Gates, Brian J, Huffman, Alexander J, Larsen, Jeremy K
• Format: Patent
• Language: English
• Published: 03.11.2022
• Subjects: OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS; OPTICS; PHYSICS

The present disclosure provides a multiphoton imaging method. The method includes a) immersing a semi-submersible microscope objective in a liquid medium that is at least one of scattering or absorbing; b) directing laser light through the semi-submersible microscope objective and into the liquid medium in an image-wise manner under conditions such that multiphoton absorption by the multiphoton absorber occurs, and at least partial polymerization of the polymerizable compound occurs resulting in an article; and c) removing uncured polymerizable compound to clean the article. The liquid medium includes a polymerizable compound, a secondary component, and a multiphoton absorber. An article is also provided. The article includes a material defining one or more tortuous or arcuate channels, one or more internal architectural voids, one or more undercuts, one or more perforations, or combinations thereof, at least one of which exhibits a surface roughness of 1.0 micrometer Ra or less.