Enrichment method and system for gene target region
The present invention provides an enrichment method for a gene target region, the method comprising: (1) amplifying, by means of a specific probe, fragmented DNA including a target region, and providing a captured extension product, wherein the specific probe comprises a sequence that is complementa...
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Main Authors | , , , |
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Format | Patent |
Language | English |
Published |
13.10.2022
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Subjects | |
Online Access | Get full text |
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Summary: | The present invention provides an enrichment method for a gene target region, the method comprising: (1) amplifying, by means of a specific probe, fragmented DNA including a target region, and providing a captured extension product, wherein the specific probe comprises a sequence that is complementary to the target region of the fragmented DNA, and a 3′-end nucleotide and a 5′-end nucleotide of the probe are both modified; and (2) adding a ligase to the captured extension product provided in step (1), and providing a ligation product. The invention further provides an enrichment system for a gene target, the system being applicable to the enrichment method for a gene target region provided in the present invention. |
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Bibliography: | Application Number: US201917760939 |