MULTI-PERSPECTIVE MICROSCOPIC IMAGING GUIDED MICROINJECTION OF MICROSCOPIC OBJECTS ACROSS LARGE FIELD OF VIEWS

Techniques are described for automated microinjection of substances, such as genetic material, into microscopic objects, such as embryos. An example system includes a pressure controller, a stereoscopic imaging apparatus, a first camera, a second camera, and a computing system. The computing system...

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Bibliographic Details
Main Authors Kodandaramaiah, Suhasa B, Auch, Benjamin, Joshi, Amey S, Gohl, Daryl M, Alegria, Andrew
Format Patent
LanguageEnglish
Published 29.09.2022
Subjects
CALCULATING
COMPUTING
COUNTING
IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
PHYSICS
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Summary:Techniques are described for automated microinjection of substances, such as genetic material, into microscopic objects, such as embryos. An example system includes a pressure controller, a stereoscopic imaging apparatus, a first camera, a second camera, and a computing system. The computing system is configured to apply one or more computer vision algorithms that determine, based on image data generated by the stereoscopic imaging apparatus, first camera, and second camera, a location of a tip of a micropipette and a location of a particular microscopic object in three-dimensional space. The computing system is further configured to control the pressure controller to cause the micropipette to eject the substance from the tip of the micropipette and into the particular microscopic object.
Bibliography:Application Number: US202217656130