Microscope and Method for Forming a Microscopic Image with an Extended Depth of Field

• Main Authors: Iliopoulos, Pavlos, Cho, Gyoungil, Gaiduk, Alexander, Karanin, Ilja, Sohn, Jin Young, Seo, Cheong Soo
• Format: Patent
• Language: English
• Published: 22.09.2022
• Subjects: ELECTRIC COMMUNICATION TECHNIQUE; ELECTRICITY; OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS; OPTICS; PHYSICS; PICTORIAL COMMUNICATION, e.g. TELEVISION

The present invention concerns a method for producing a microscopic image with an extended depth of field by means of a microscope. The microscope comprises an images sensor that comprises pixels that are arranged as a matrix that is formed by lines. In a step of the method, a plurality of microscopic frames of a specimen is acquired while a focus position (z) is changed. The microscopic frames are acquired line by line. The focus position (z) is changed over a course of acquiring individuals of the microscopic frames. In a further step, parts of individuals of the acquired lines are identified. These parts sharply image the specimen. The identified parts of the lines are composed in order to form a microscopic image of the specimen with an extended depth of field. Furthermore, the present invention concerns a microscope.