A METHOD FOR RECOMBINANT PROTEIN PRODUCTION

A method including the process steps of insertion of a gene to be expressed into the plasmid carrying AOX1 promoter (i), cloning of plasmid carrying gene (ii), transfer of recombinant plasmid carrying AOX1 promoter and gene to expression host (iii) and providing both induction of AOX1 promoter and e...

Full description

Saved in:
Bibliographic Details
Main Authors ÜNVER, Yagmur, ACAR, Melek, YILDIZ, Seyda, TASKIN, Mesut, YILDIZ, Melike
Format Patent
LanguageEnglish
Published 14.07.2022
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online AccessGet full text

Cover

More Information
Summary:A method including the process steps of insertion of a gene to be expressed into the plasmid carrying AOX1 promoter (i), cloning of plasmid carrying gene (ii), transfer of recombinant plasmid carrying AOX1 promoter and gene to expression host (iii) and providing both induction of AOX1 promoter and expression of associated heterologous gene, using a nitric oxide donor (iv). The method enables expression of various genes of various microorganisms, plants, animals and human, and thus extracellular and/or intracellular production of various recombinant proteins.
Bibliography:Application Number: US202017607185