MARKER PEPTIDE OF SNAKE VENOM THROMBIN-LIKE ENZYMES (SVTLES) FROM AGKISTRODON HALYS PALLAS AND APPLICATION THEREOF

• Main Authors: Nie, Li, Hang, Baojian, Shi, Feng, Chi, Lianli, Gong, Liping, Wang, Weijian, Wang, Fengshan, Xian, Ruiqing, Wang, Congcong, Zhang, Xunjie
• Format: Patent
• Language: English
• Published: 03.02.2022
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The present invention relates to the field of chemical analysis detection and application, in particular to a marker peptide of snake venom thrombin-like enzymes (SVTLEs) from Agkistrodon Halys Pallas and an application thereof. The amino acid sequence of the marker peptide of snake venom thrombin-like enzymes (SVTLEs) from Agkistrodon Halys Pallas is TLCAGVMEGGIDTCNR. Characterizing the source of species and a content of the SVTLEs in a to-be-detected sample by using the marker peptide includes the following steps of: pretreating the to-be-detected sample by trypsin through enzymolysis, and taking a supernatant of an enzymolysis liquid as a test solution; and injecting the test solution and a reference solution into a liquid chromatography-mass spectrometer, and selecting a qualitative ion pair and a quantitative ion pair for detecting the source of species and a content of the SVTLEs in the to-be-detected sample.