GENOMIC EDITING VECTOR FOR EUBACTERIUM CALLANDERI, METHOD FOR EDITING GENOME OF EUBACTERIUM CALLANDERI USING THE SAME, AND TRANSGENIC EUBACTERIUM CALLANDERI STRAINS USING THE SAME

Proposed is a genomic editing vector for Eubacterium callanderi for the CRISPR/Cas9 system. The genomic editing vector for Eubacterium callanderi includes a DNA sequence encoding a guide RNA (gRNA) of a cleavage target gene; a DNA sequence encoding a Cas9 protein; a Prbo promoter that is operably li...

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Bibliographic Details
Main Authors JEONG, Ji Yeong, KIM, Ji Yeon, CHANG, In Seop, OH, So Young, JEON, Jin Sung
Format Patent
LanguageEnglish
Published 20.01.2022
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Proposed is a genomic editing vector for Eubacterium callanderi for the CRISPR/Cas9 system. The genomic editing vector for Eubacterium callanderi includes a DNA sequence encoding a guide RNA (gRNA) of a cleavage target gene; a DNA sequence encoding a Cas9 protein; a Prbo promoter that is operably linked to the DNA sequence encoding a Cas9 protein; a replication starting point derived from E. coli; a replication starting point for Eubacterium callanderi; and a marker for selecting transgenic strains. It is possible to provide a genomic editing vector for Eubacterium callanderi that can be applied to Eubacterium callanderi strains that are acetogen that is suitable for syngas biorefinery, a method for editing a genome of Eubacterium callanderi strains using the same, and transgenic Eubacterium callanderi strains using the same.
Bibliography:Application Number: US202117377590