DUAL GUIDE RNA FOR CRISPR/CAS GENOME EDITING IN PLANTS CELLS

The invention pertains to a method for targeted modification of DNA in a plant cell, comprising a step of contacting the DNA with an RNA-guided CRISPR-system nuclease complex, wherein the complex comprises a crRNA and a tracrRNA as separate molecules. The invention further pertains to said RNA-guide...

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Bibliographic Details
Main Author LHUISSIER, Franck Georges Paul
Format Patent
LanguageEnglish
Published 13.01.2022
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention pertains to a method for targeted modification of DNA in a plant cell, comprising a step of contacting the DNA with an RNA-guided CRISPR-system nuclease complex, wherein the complex comprises a crRNA and a tracrRNA as separate molecules. The invention further pertains to said RNA-guided CRISPR-system nuclease complex for targeting of DNA in a plant cell and kits comprising the RNA-guided CRISPR-system nuclease complex or constructs encoding the same.
Bibliography:Application Number: US201917290547