POLYNUCLEOTIDE DUPLEX PROBE MOLECULE

• Main Authors: LIGHTWOOD, Daniel John, WRIGHT, Michael John
• Format: Patent
• Language: English
• Published: 26.08.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present disclosure relates to a duplex probe molecule comprising: i) a double-stranded-core (the core) comprising a first polynucleotide strand (first-strand) and a second polynucleotide strand (second-strand), wherein the first and second strands are complementary to each other, ii) a single stranded first polynucleotide probe (first probe) sequence extending in the 5′ to 3′ direction from the first-strand of the core; and iii) single stranded second polynucleotide probe (second probe) sequence extending in the 5′ to 3′ direction from the second strand of the core; wherein the first and second probes extend outwards from the double stranded core in opposing directions on different polynucleotide strands and each terminate in a 3′ end. The disclosure also extends to methods of making the molecule and use of the molecule, for example to capture variable domains sequence information from antibodies.