PRIMER, KIT AND METHOD FOR DETECTING TAIHE BLACK CHICKEN EGG

• Main Authors: Zhou, Min, Ma, Jing'e, Gong, Jishang, Chen, Zhiwu, Xu, Jiguo, Zhu, Xuenong, Chen, Tingchong, Yang, Yanbei, Xu, Qiao, Wang, Zhangfeng, Tan, Yuwen, Li, Yuanfei, Peng, Jianjun, Rao, Yousheng, Xiong, Xinwei
• Format: Patent
• Language: English
• Published: 24.06.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

This invention discloses a primer, a kit and a method for testing Taihe black chicken egg. The described primer includes: as shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 4. The described test method comprises the following steps: extract the genomic DNA of the egg to be tested, propagate the genomic DNA by two-step PCR, and perform genotyping according to the base at 68 bp of the gene sequence of the second-step PCR product. If the base at that site is G, the egg is a Taihe black chicken egg; if the base at that site is C, the egg is a non-Taihe black chicken egg. The variable site targeted by this invention is unique to Taihe black chicken egg.