PRIMER, KIT AND METHOD FOR DETECTING TAIHE BLACK CHICKEN EGG

This invention discloses a primer, a kit and a method for testing Taihe black chicken egg. The described primer includes: as shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 4. The described test method comprises the following steps: extract the genomic DNA of the egg to be tested, p...

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Main Authors Zhou, Min, Ma, Jing'e, Gong, Jishang, Chen, Zhiwu, Xu, Jiguo, Zhu, Xuenong, Chen, Tingchong, Yang, Yanbei, Xu, Qiao, Wang, Zhangfeng, Tan, Yuwen, Li, Yuanfei, Peng, Jianjun, Rao, Yousheng, Xiong, Xinwei
Format Patent
LanguageEnglish
Published 24.06.2021
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:This invention discloses a primer, a kit and a method for testing Taihe black chicken egg. The described primer includes: as shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 4. The described test method comprises the following steps: extract the genomic DNA of the egg to be tested, propagate the genomic DNA by two-step PCR, and perform genotyping according to the base at 68 bp of the gene sequence of the second-step PCR product. If the base at that site is G, the egg is a Taihe black chicken egg; if the base at that site is C, the egg is a non-Taihe black chicken egg. The variable site targeted by this invention is unique to Taihe black chicken egg.
Bibliography:Application Number: US202017122055