PROTEINASE-FREE COATINGS FOR COLONY PASSAGING

A cell culture article includes a substrate having a polymer coating that is conducive to colony passaging of cells cultured on the coating. Example polymer coatings are formed from polygalacturonic acid (PGA), alginate, or combinations thereof. Cells cultured on the polymer coating can be separated...

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Bibliographic Details
Main Authors Fang, Ye, Zhou, Yue, Henry, David, Goral, Vasiliy Nikolaevich, Walerack, Corinne, Ferrie, Ann MeeJin, Hervy, Martial
Format Patent
LanguageEnglish
Published 04.03.2021
Subjects
ADHESIVES
AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F,C08G
BEER
BIOCHEMISTRY
CHEMICAL PAINT OR INK REMOVERS
CHEMISTRY
COATING COMPOSITIONS, e.g. PAINTS, VARNISHES ORLACQUERS
COMPOSITIONS BASED THEREON
COMPOSITIONS THEREOF
CORRECTING FLUIDS
CULTURE MEDIA
DYES
ENZYMOLOGY
FILLING PASTES
GENERAL PROCESSES OF COMPOUNDING
INKS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MISCELLANEOUS APPLICATIONS OF MATERIALS
MISCELLANEOUS COMPOSITIONS
MUTATION OR GENETIC ENGINEERING
NATURAL RESINS
ORGANIC MACROMOLECULAR COMPOUNDS
PAINTS
PASTES OR SOLIDS FOR COLOURING OR PRINTING
POLISHES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
THEIR PREPARATION OR CHEMICAL WORKING-UP
USE OF MATERIALS THEREFOR
VINEGAR
WINE
WOODSTAINS
WORKING-UP
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Summary:A cell culture article includes a substrate having a polymer coating that is conducive to colony passaging of cells cultured on the coating. Example polymer coatings are formed from polygalacturonic acid (PGA), alginate, or combinations thereof. Cells cultured on the polymer coating can be separated from the substrate as a colony or layer of cells by exposing the polymer coating to (i) a chelating agent, (ii) a proteinase-free enzyme, or (iii) a chelating agent and a proteinase-free enzyme.
Bibliography:Application Number: US202017022764