ORGANELLE GENOME MODIFICATION USING POLYNUCLEOTIDE GUIDED ENDONUCLEASE

Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide...

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Bibliographic Details
Main Authors Sakai, Hajime, Kishore, Ganesh, Yoo, Byung-Chun, Orozco, Jr., Emil Meyer, Wyse, Roger, Yadav, Narendra Singh, Keasling, Jay
Format Patent
LanguageEnglish
Published 25.02.2021
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.
Bibliography:Application Number: US201816641073