NUCLEOTIDE SEQUENCING ELEMENT AND CHIP, AND SEQUENCING ANALYSIS METHOD

• Main Authors: SHIAU, Jeng-Huei, LIEN, Chun-Lung
• Format: Patent
• Language: English
• Published: 04.02.2021
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; TESTING; VINEGAR; WINE

Provided is a nucleotide sequencing element, comprising a semiconductor substrate, a transistor, a dielectric layer, an annular electrode group, and a conductor. Also provided is a nucleotide sequencing chip, comprising the nucleotide sequencing element and a sense amplifier. Also provided is a sequencing analysis method, comprising: providing the nucleotide sequencing chip as stated above; placing a nucleotide sequence fragment to be tested and a known nucleotide sequence fragment in a slot separately for a polymerization reaction and an electrochemical reaction; and analyzing the electrical signals generated by the reactions to obtain a sequencing result.